14-3-3 GAMMA-Specific Polyklonaler Antikörper

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• KD/KO Validated

14-3-3 GAMMA-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 12381-1-AP

Synonyme

14-3-3 GAMMA, YWHAG, 14-3-3 protein gamma, 14-3-3, 14 3 3GAMMA

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Galerie der Validierungsdaten

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  WB analysis using 12381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse IMCD3 cells (RNAi) using 12381-1-AP

  siRNA result of 12381-1-AP (anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo.

• 

  WB analysis of A431 using 12381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12381-1-AP

  IP result of anti-14-3-3 GAMMA-Specific (IP:12381-1-AP, 3ug; Detection:12381-1-AP 1:800) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human lung cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 12381-1-AP

  Immunofluorescent analysis of MDCK cells using 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-IMCD3-Zellen (RNAi), A431-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

12381-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA 14-3-3 GAMMA-Specific und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: 14-3-3 GAMMA-Specific fusion protein Ag3047
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28-33 kDa
• GenBank-Zugangsnummer: BC020963
• Gene symbol: 14-3-3 gamma
• Gene ID (NCBI): 7532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.

Publikationen

Species	Application	Title
human	CoIP,WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Human adipocyte differentiation and composition of disease-relevant lipids are regulated by miR-221-3p.; Authors - Maria A Ahonen
human	WB	Proteomics; Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.; Authors - Songlin Zeng
human	WB,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song
human	CoIP	Autophagy; YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.; Authors - Yang Xu
rat	WB	J Biochem Mol Toxicol; miR-200a-3p inhibits the PDGF-BB-induced proliferation of VSMCs by affecting their phenotype-associated proteins; Authors - Rui-Yuan Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erik (Verified Customer) (08-26-2021)

We used the antibody at 1:1000 dilution with 11ug input protein, and it produced one clean and strong band at the expected height for the protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MIAPACA2 and PANC1 cell lines

14-3-3 GAMMA-Specific Antibody Western Blot validation (1:1000 dilution) in MIAPACA2 and PANC1 cell lines (Cat no:12381-1-AP)