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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human GDNF protein

Activity(Primary)

< 10 ng/mL

Specific Activity

minimally 1.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1311

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Validation Data Gallery

  • Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

    GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

  • Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

    Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

  • Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

    Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

Technical Specifications

GeneID 2668
Species Human
Expression HEK293
Activity(Primary) < 10 ng/mL
Specific Activityminimally 1.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/g
Accession NumberP39905
Molecular Mass 22 to 25 kDa reduced, 30 to 38 kDa non-reduced, homodimer,glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

N/A

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Publications

SpeciesTitle

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Grace (Verified Customer) (09-13-2024)

I reconstitute the GDNF in 1x PBS supplemented with 0.1% BSA (to help stabilize in solution) and use in the media to differentiate ipsc to cortical neurons. I really like that this product in made in HEK cells not e.coli.

  • Applications: Cell culture
  • Cell Tissue Type: ipsc derived neurons
FH

Ankush (Verified Customer) (02-19-2024)

I used GDNF for brain organoid generation and it worked great

  • Applications: Cell culture
  • Cell Tissue Type: Midbrain Organoid
FH

Alessandro (Verified Customer) (07-27-2022)

Reliable supplement for dopaminergic neurons maturation

  • Applications: Cell culture
  • Cell Tissue Type: Dopaminergic Neurons