ZP3 Polyclonal antibody

ZP3 Polyclonal Antibody for IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21279-1-AP

Synonyms

ZP3A/ZP3B, ZP3A, Zp-3, Zp 3, Zona pellucida sperm-binding protein 3



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse ovary tissue

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21279-1-AP targets ZP3 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZP3 fusion protein Ag15791
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Calculated Molecular Weight 373 aa, 41 kDa
Observed Molecular Weight 47 kDa, 60 kDa
GenBank Accession NumberBC113949
Gene Symbol ZP3
Gene ID (NCBI) 7784
RRIDAB_11124319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).

Protocols

Product Specific Protocols
IHC protocol for ZP3 antibody 21279-1-APDownload protocol
IF protocol for ZP3 antibody 21279-1-APDownload protocol
IP protocol for ZP3 antibody 21279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

Cell Death Differ

Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.

Authors - Zhaokang Cui
humanIHC,IF,CoIP

Am J Hum Genet

A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility.

Authors - Tailai Chen
hamsterIF

Fertil Steril

The critical role of ZP genes in female infertility characterized by empty follicle syndrome and oocyte degeneration.

Authors - Ping Yang
mouseWB,IF

Hum Reprod

Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.

Authors - Ying Shen
humanIF

Cell Discov

Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance.

Authors - Chunfang Liu
mouseIF

Hum Mutat

A novel gene mutation in ZP3 loop region identified in patients with empty follicle syndrome

Authors - Zhiqiang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Julia (Verified Customer) (08-18-2022)

It works well.

  • Applications: Western Blot, Immunofluorescence
FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works beautifully to highlight the zona pellucida on mouse ovary sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse ovary sections
ZP3 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:21279-1-AP)