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  • KD/KO Validated

ZNF143 Polyclonal antibody

ZNF143 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16618-1-AP

Synonyms

hStaf, pHZ 1, SBF, SPH binding factor, STAF, zinc finger protein 143, ZNF143



Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16618-1-AP targets ZNF143 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF143 fusion protein Ag9914
Full Name zinc finger protein 143
Calculated Molecular Weight 626 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC020219
Gene Symbol ZNF143
Gene ID (NCBI) 7702
RRIDAB_2218324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8

Protocols

Product Specific Protocols
WB protocol for ZNF143 antibody 16618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousechIP

Mol Cell

Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo.

Authors - Yoon Hee Jung
humanchIP

Mol Cell

Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress.

Authors - Xiaowen Lyu
mouseCoIP

Nat Commun

Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.

Authors - Tiffany Chern
humanChIP

Proc Natl Acad Sci U S A

Cooperative transcription factor associations discovered using regulatory variation.

Authors - Karczewski Konrad J KJ
humanchIP

Cancer Res

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Authors - Lili Zhang
  • KD Validated
humanWB, ChIP

Cell Rep

Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.

Authors - J Brandon Parker
  • KD Validated