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- KD/KO Validated
ZNF143 Polyclonal antibody
ZNF143 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 16618-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
| ChIP | See 8 publications below |
Product Information
16618-1-AP targets ZNF143 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZNF143 fusion protein Ag9914 |
| Full Name | zinc finger protein 143 |
| Calculated Molecular Weight | 626 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC020219 |
| Gene Symbol | ZNF143 |
| Gene ID (NCBI) | 7702 |
| RRID | AB_2218324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF143 antibody 16618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo. | ||
Mol Cell Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress. | ||
Nat Commun Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. | ||
Proc Natl Acad Sci U S A Cooperative transcription factor associations discovered using regulatory variation. | ||
Cancer Res ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.
| ||
Cell Rep Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.
|
