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ZC3H14 Polyclonal antibody

ZC3H14 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 28190-1-AP

Synonyms

UKp68, NY REN 37

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZC3H14 Polyclonal antibody (28190-1-AP)

    WB analysis of mouse brain using 28190-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28190-1-AP (ZC3H14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using ZC3H14 Polyclonal antibody (28190-1-AP)

    IP experiment of HEK-293T using 28190-1-AP

    IP result of anti-ZC3H14 (IP:28190-1-AP, 4ug; Detection:28190-1-AP 1:4000) with HEK-293T cells lysate 2200 ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28190-1-AP targets ZC3H14 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3H14 fusion protein Ag28182
Full Name zinc finger CCCH-type containing 14
Calculated Molecular Weight 83 aa
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_001160103
Gene Symbol ZC3H14
Gene ID (NCBI) 79882
RRIDAB_2881085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZC3H14 antibody 28190-1-APDownload protocol
IP protocol for ZC3H14 antibody 28190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols