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ZBTB32 Polyclonal antibody

ZBTB32 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12516-1-AP

Synonyms

ZNF538, Zinc finger protein 538, Zinc finger and BTB domain-containing protein 32, FAZF, FAXF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZBTB32 Polyclonal antibody (12516-1-AP)

    WB analysis using 12516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12516-1-AP (ZBTB32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ZBTB32 Polyclonal antibody (12516-1-AP)

    IHC staining of mouse testis using 12516-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ZBTB32 Polyclonal antibody (12516-1-AP)

    IHC staining of mouse testis using 12516-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZBTB32 Polyclonal antibody (12516-1-AP)

    IF Staining of MCF-7 using 12516-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 21738-1-AP 00069062 (red).

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue, pig lymph node tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12516-1-AP targets ZBTB32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB32 fusion protein Ag3199
Full Name zinc finger and BTB domain containing 32
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017700
Gene Symbol ZBTB32
Gene ID (NCBI) 27033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The zinc finger and BTB domain containing 32 (ZBTB32) protein, also known as testis zinc finger protein (TZFP) or repressor of GATA (ROG), is a transcription factor belonging to the BTB/POZ-ZF protein family. It is encoded by the Zbtb32 gene, located on proximal chromosome 7 in the mouse genome. ZBTB32 was shown to ensure proper regulation of meiosis during spermatogenesis by acting as a repressor of the androgen receptor (PMID: 22851713, 34337361).

Protocols

Product Specific Protocols
WB protocol for ZBTB32 antibody 12516-1-APDownload protocol
IHC protocol for ZBTB32 antibody 12516-1-APDownload protocol
IF protocol for ZBTB32 antibody 12516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols