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Villin like Polyclonal antibody

Villin like Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15130-1-AP

Synonyms

VILL, Villin-like protein, Villin like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Villin like Polyclonal antibody (15130-1-AP)

    WB analysis using 15130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15130-1-AP (VILL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IHC staining of mouse small intestine using 15130-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15130-1-AP (VILL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of mouse small intestine using 15130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using Villin like Polyclonal antibody (15130-1-AP)

    IF Staining of rat small intestine using 15130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected in37°C incubated mouse colon tissue, rat colon
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, rat small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15130-1-AP targets Villin like in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Villin like fusion protein Ag7259
Full Name villin-like
Calculated Molecular Weight 96 kDa
Observed Molecular Weight96-100 kDa
GenBank Accession NumberBC000243
Gene Symbol VILL
Gene ID (NCBI) 50853
RRIDAB_2918038
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Villin like antibody 15130-1-APDownload protocol
IHC protocol for Villin like antibody 15130-1-APDownload protocol
IF protocol for Villin like antibody 15130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols