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  • Featured Product
  • KD/KO Validated

TXNRD1 Polyclonal antibody

TXNRD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11117-1-AP

Synonyms

TRXR1, GRIM12, GRIM 12, Gene associated with retinoic and interferon-induced mortality 12 protein, Gene associated with retinoic and IFN-induced mortality 12 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNRD1 Polyclonal antibody (11117-1-AP)

    WB analysis using 11117-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TXNRD1 Polyclonal antibody (11117-1-AP)

    WB analysis of HeLa using 11117-1-AP

    WB result of TXNRD1 antibody (11117-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNRD1 transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using TXNRD1 Polyclonal antibody (11117-1-AP)

    WB analysis of MCF-7 using 11117-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TXNRD1 Polyclonal antibody (11117-1-AP)

    WB analysis of Jurkat using 11117-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of human testis using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of mouse testis using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of human breast cancer using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of human breast cancer using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of mouse testis using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TXNRD1 Polyclonal antibody (11117-1-AP)

    IHC staining of mouse testis using 11117-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXNRD1 Polyclonal antibody (11117-1-AP)

    IF Staining of HeLa using 11117-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNRD1 antibody (11117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11117-1-AP targets TXNRD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNRD1 fusion protein Ag1618
Full Name thioredoxin reductase 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC018122
Gene Symbol TXNRD1
Gene ID (NCBI) 7296
RRIDAB_2210113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Protocols

Product Specific Protocols
WB protocol for TXNRD1 antibody 11117-1-APDownload protocol
IHC protocol for TXNRD1 antibody 11117-1-APDownload protocol
IF protocol for TXNRD1 antibody 11117-1-APDownload protocol
FC protocol for TXNRD1 antibody 11117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Redox Biol

Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.

Authors - Angel Gallego-Selles
View Article
human,mouseWB

J Immunother Cancer

Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.

Authors - Weinan Guo
View Article
humanWB

Cell Death Dis

Elovanoid-N34 modulates TXNRD1 key in protection against oxidative stress-related diseases

Authors - Jorgelina M Calandria
View Article
humanWB

Cancer Res

Natural allelic variations in glutathione peroxidase-1 affect it subcellular localization and function.

Authors - Soumen Bera
View Article
ratWB

Nucleic Acids Res

Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression.

Authors - Miniard Angela C AC
View Article
humanWB

Biomed Pharmacother

TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling

Authors - Yifan Chen
View Article