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TRNT1 Polyclonal antibody

TRNT1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25376-1-AP

Synonyms

MTO2, MTO2 homolog, MTU1, TRMT, TRMT1, TRMU, TRNT1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TRNT1 Polyclonal antibody (25376-1-AP)

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TRNT1 Polyclonal antibody (25376-1-AP)

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRNT1 Polyclonal antibody (25376-1-AP)

    IF Staining of HepG2 using 25376-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25376-1-AP (TRMU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25376-1-AP targets TRNT1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRNT1 fusion protein Ag21930
Full Name tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Calculated Molecular Weight 421 aa, 48 kDa
GenBank Accession NumberBC027991
Gene Symbol TRNT1
Gene ID (NCBI) 55687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TRNT1 antibody 25376-1-APDownload protocol
IF protocol for TRNT1 antibody 25376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols