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TOM70 Polyclonal antibody
TOM70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14528-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, mouse heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 65 publications below |
| IHC | See 1 publications below |
| IF | See 13 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14528-1-AP targets TOM70 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOM70 fusion protein Ag6027 |
| Full Name | translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC052994 |
| Gene Symbol | TOM70 |
| Gene ID (NCBI) | 9868 |
| RRID | AB_2303727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOM70 antibody 14528-1-AP | Download protocol |
| IHC protocol for TOM70 antibody 14528-1-AP | Download protocol |
| IF protocol for TOM70 antibody 14528-1-AP | Download protocol |
| IP protocol for TOM70 antibody 14528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Nature In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. | ||
Nature Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tom (Verified Customer) (04-15-2021) | Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.
 |
FH Daniela (Verified Customer) (09-10-2019) | Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.
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