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LIGHT Polyclonal antibody

LIGHT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 14194-1-AP

Synonyms

CD258, HVEM L, HVEML, LIGHT, LTg, TNFSF14, TR2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human spleen tissue using LIGHT Polyclonal antibody (14194-1-AP)

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LIGHT Polyclonal antibody (14194-1-AP)

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14194-1-AP targets LIGHT in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIGHT fusion protein Ag5380
Full Name tumor necrosis factor (ligand) superfamily, member 14
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC018058
Gene Symbol LIGHT
Gene ID (NCBI) 8740
RRIDAB_2256332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

Protocols

Product Specific Protocols
IHC protocol for LIGHT antibody 14194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols