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Tested Applications
| Positive WB detected in | BxPC-3 cells, HEK-293T cells, Neuro-2a cells, PC-3 cells, Raji cells, U-87 MG cells, NIH/3T3 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | mouse brain tissue, human colon cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15199-1-AP targets TMP21 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMP21 fusion protein Ag7347 Predict reactive species |
| Full Name | transmembrane emp24-like trafficking protein 10 (yeast) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC001825 |
| Gene Symbol | TMED10 |
| Gene ID (NCBI) | 10972 |
| RRID | AB_2204321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49755 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMP21 antibody 15199-1-AP | Download protocol |
| IHC protocol for TMP21 antibody 15199-1-AP | Download protocol |
| IP protocol for TMP21 antibody 15199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
| ||
Nat Cell Biol A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion | ||
Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. | ||
Brain Behav Immun Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis | ||
J Cell Sci A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH pitna (Verified Customer) (12-19-2019) | Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions
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FH Boyan (Verified Customer) (01-25-2019) | Good. By IF, PFA fixation, it labels the protein at the Golgi.
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