Validation Data Gallery
|Positive WB detected in||BxPC-3 cells, HEK-293T cells, Neuro-2a cells, PC-3 cells, Raji cells, U-87 MG cells, NIH/3T3 cells, mouse pancreas tissue, rat pancreas tissue|
|Positive IHC detected in||mouse brain tissue, human colon cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15199-1-AP targets TMP21 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMP21 fusion protein Ag7347|
|Full Name||transmembrane emp24-like trafficking protein 10 (yeast)|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC001825|
|Gene ID (NCBI)||10972|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
J Cell Sci
A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.
The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.
Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.
Adv Sci (Weinh)
TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
pitna (Verified Customer) (12-19-2019)
Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions
Boyan (Verified Customer) (01-25-2019)
Good. By IF, PFA fixation, it labels the protein at the Golgi.