• Featured Product
  • KD/KO Validated

TMP21 Polyclonal antibody

TMP21 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15199-1-AP

Synonyms

P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21



Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293T cells, Neuro-2a cells, PC-3 cells, Raji cells, U-87 MG cells, NIH/3T3 cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15199-1-AP targets TMP21 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMP21 fusion protein Ag7347
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC001825
Gene Symbol TMED10
Gene ID (NCBI) 10972
RRIDAB_2204321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMP21 antibody 15199-1-APDownload protocol
IHC protocol for TMP21 antibody 15199-1-APDownload protocol
IF protocol for TMP21 antibody 15199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated
humanWB

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
humanCoIP

J Cell Sci

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Authors - Jiaxin Sun
humanWB,IF

BMC Cancer

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Authors - Xue Yang
mouseWB

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
humanWB

Adv Sci (Weinh)

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Authors - Hak Park

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)
TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)
FH

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

  • Applications: Immunofluorescence,