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  • Featured Product
  • KD/KO Validated

Thrombospondin 3-Specific Polyclonal antibody

Thrombospondin 3-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19727-1-AP

Synonyms

THBS3, thrombospondin 3, Thrombospondin 3-Specific, TSP3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of human liver using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of human liver using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of mouse kidney using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of mouse kidney using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of human colon using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of human colon using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Thrombospondin 3-Specific Polyclonal antibody (19727-1-AP)

    IHC staining of human colon using 19727-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman liver tissue, human colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19727-1-AP targets Thrombospondin 3-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name thrombospondin 3
Calculated Molecular Weight 104 kDa
GenBank Accession NumberNM_007112
Gene Symbol Thrombospondin 3
Gene ID (NCBI) 7059
RRIDAB_10695895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THBS3, also named as TSP3, belongs to the thrombospondin family. THBS3 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. THBS3 can bind to fibrinogen, fibronectin, laminin and type V collagen. The antibody is specific to THBS3.

Protocols

Product Specific Protocols
IHC protocol for Thrombospondin 3-Specific antibody 19727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.

Authors - Davy Vanhoutte
View Article
humanIF

Front Cell Dev Biol

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Authors - Mansheng Li
View Article
mouseWB,IF

Nat Commun

Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.

Authors - Tobias G Schips
  • KO Validated
View Article