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TGF Beta 1 Polyclonal antibody
TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment
Conjugate
Unconjugated
654
Cat no : 21898-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skin tissue, A549 cells, HeLa cells, K-562 cells, L-929 cells, mouse spleen tissue, rat skin tissue |
| Positive IF-P detected in | human stomach cancer tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 520 publications below |
| IHC | See 146 publications below |
| IF | See 69 publications below |
| IP | See 1 publications below |
| ELISA | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
21898-1-AP targets TGF Beta 1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, zebrafish, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGF Beta 1 fusion protein Ag13591 |
| Full Name | transforming growth factor, beta 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000125 |
| Gene Symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| RRID | AB_2811115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).
This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| IF protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Acta Pharm Sin B Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment. | ||
Dev Cell Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation | ||
Redox Biol Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Andrea (Verified Customer) (03-22-2024) | We had a good confocal signal and beautiful bands in our experimental group of high TGFb
|
FH Kenzo (Verified Customer) (08-15-2023) | This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.
|
FH Celina (Verified Customer) (07-31-2023) | antibody worked well for IF of human cardiac ventricular fibroblasts
|
FH Udesh (Verified Customer) (12-06-2022) | The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.
 |
FH Yasuyo (Verified Customer) (05-24-2022) | Works great!!!
 |
FH P (Verified Customer) (12-01-2021) | Excellent
 |
FH Iram (Verified Customer) (09-04-2020) | Very clean bands for western
|
FH Qingyuan (Verified Customer) (11-05-2019) | The antibody clearly shows one band with the right molecular weight size.
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