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Transferrin/TF Polyclonal antibody
Transferrin/TF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17435-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human urine sample, mouse kidney tissue, mouse spleen tissue, rat kidney tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 51 publications below |
| IHC | See 9 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
Product Information
17435-1-AP targets Transferrin/TF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Transferrin/TF fusion protein Ag11504 |
| Full Name | transferrin |
| Calculated Molecular Weight | 698 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC059367 |
| Gene Symbol | TF |
| Gene ID (NCBI) | 7018 |
| RRID | AB_2035023 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
77 kDa
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Transferrin/TF antibody 17435-1-AP | Download protocol |
| IHC protocol for Transferrin/TF antibody 17435-1-AP | Download protocol |
| IF protocol for Transferrin/TF antibody 17435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation. | ||
Sci Adv The adipocyte-enriched secretory protein tetranectin exacerbates type 2 diabetes by inhibiting insulin secretion from β cells | ||
Research (Wash D C) ATP5O Hypo-crotonylation Caused by HDAC2 Hyper-Phosphorylation Is a Primary Detrimental Factor for Downregulated Phospholipid Metabolism under Chronic Stress | ||
Biomaterials The protein corona hampers the transcytosis of transferrin-modified nanoparticles through blood-brain barrier and attenuates their targeting ability to brain tumor. | ||
Haematologica Gastrointestinal iron excretion and reversal of iron excess in a mouse model of inherited iron excess.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Aditya (Verified Customer) (11-01-2024) | great with icc
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