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SPOPL Polyclonal antibody

SPOPL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17740-1-AP

Synonyms

HIB homolog 2, Roadkill homolog 2, speckle type POZ protein like, SPOPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPOPL Polyclonal antibody (17740-1-AP)

    WB analysis using 17740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17740-1-AP (SPOPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17740-1-AP targets SPOPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPOPL fusion protein Ag11951
Full Name speckle-type POZ protein-like
Calculated Molecular Weight 392 aa, 45 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC071613
Gene Symbol SPOPL
Gene ID (NCBI) 339745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Speckle Type POZ Protein Like (SPOPL), a member of the MATH-BTB protein family, was frst identifed in 2010 with 82.6% sequence homology with Speckle-type POZ protein (SPOP). SPOPL is an E3 ubiquitin ligase protein that ubiquitinates and degrades the target protein. SPOPL can be used as a potential prognostic biomarker for glioblastoma multiforme in clinical work and promotes the proliferation and stemness of glioma stem cells by activating the Notch signaling pathway (PMID:37523046).

Protocols

Product Specific Protocols
WB protocol for SPOPL antibody 17740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols