- Featured Product
- KD/KO Validated
SOD1 Polyclonal antibody
SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
275
Cat no : 10269-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 247 publications below |
| IHC | See 40 publications below |
| IF | See 15 publications below |
| IP | See 3 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOD1 fusion protein Ag0335 |
| Full Name | superoxide dismutase 1, soluble |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC001034 |
| Gene Symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| RRID | AB_2193750 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IHC protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IF protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IP protocol for SOD1 antibody 10269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons | ||
Bioact Mater Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Nat Commun Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis. | ||
Microbiome Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Scott (Verified Customer) (10-22-2024) | 20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373. First 5 lanes are Transgenic SOD1G93A mice, last four lanes are non-transgenic mice.
 |
FH Tianyi (Verified Customer) (08-17-2023) | Paraffin-embedded tissues (4% PFA, 24 h) incubated with SOD1 (1:200) and secondary antibody Alexa Fluor donkey anti-rabbit 594 IgG (H+L).
 |
FH Xingyuan (Verified Customer) (07-17-2023) | Quality was decent.
 |
FH Macarena Lucia (Verified Customer) (10-17-2022) | clear band.
|
FH Elise (Verified Customer) (06-24-2022) | Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %
|
FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.
|
