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SH3RF2 Polyclonal antibody

SH3RF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13306-1-AP

Synonyms

POSH-eliminating RING protein, POSH3, HEPP1, Heart protein phosphatase 1-binding protein, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3RF2 Polyclonal antibody (13306-1-AP)

    WB analysis using 13306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13306-1-AP (SH3RF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SH3RF2 Polyclonal antibody (13306-1-AP)

    IHC staining of human colon cancer using 13306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13306-1-AP (SH3RF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SH3RF2 Polyclonal antibody (13306-1-AP)

    IHC staining of human colon cancer using 13306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13306-1-AP (SH3RF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SH3RF2 Polyclonal antibody (13306-1-AP)

    IF Staining of Caco-2 using 13306-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SH3RF2 antibody (13306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13306-1-AP targets SH3RF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3RF2 fusion protein Ag4127
Full Name SH3 domain containing ring finger 2
Calculated Molecular Weight 729 aa, 79 kDa
GenBank Accession NumberBC031650
Gene Symbol SH3RF2
Gene ID (NCBI) 153769
RRIDAB_2918032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SH3RF2 antibody 13306-1-APDownload protocol
IHC protocol for SH3RF2 antibody 13306-1-APDownload protocol
IF protocol for SH3RF2 antibody 13306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols