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RPA4 Polyclonal antibody

RPA4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18144-1-AP

Synonyms

HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPA4 Polyclonal antibody (18144-1-AP)

    WB analysis of HeLa using 18144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using RPA4 Polyclonal antibody (18144-1-AP)

    WB analysis of mouse spleen using 18144-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPA4 Polyclonal antibody (18144-1-AP)

    IP experiment of HeLa using 18144-1-AP

    IP result of anti-RPA4 (IP:18144-1-AP, 4ug; Detection:18144-1-AP 1:300) with HeLa cells lysate 3400ug.

Tested Applications

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18144-1-AP targets RPA4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA4 fusion protein Ag12800
Full Name replication protein A4, 34kDa
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC069791
Gene Symbol RPA4
Gene ID (NCBI) 29935
RRIDAB_2180970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)

Protocols

Product Specific Protocols
WB protocol for RPA4 antibody 18144-1-APDownload protocol
IP protocol for RPA4 antibody 18144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols