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RPA3 Polyclonal antibody

RPA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10692-1-AP

Synonyms

REPA3, Replication factor A protein 3, replication protein A3, 14kDa, RF A protein 3, RP A p14, RPA14, RPA3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPA3 Polyclonal antibody (10692-1-AP)

    WB analysis using 10692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10692-1-AP (RPA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RPA3 Polyclonal antibody (10692-1-AP)

    IHC staining of human lung cancer using 10692-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10692-1-AP (RPA3 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RPA3 Polyclonal antibody (10692-1-AP)

    IHC staining of human lung cancer using 10692-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10692-1-AP (RPA3 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, A549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10692-1-AP targets RPA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA3 fusion protein Ag1065
Full Name replication protein A3, 14kDa
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC005264
Gene Symbol RPA3
Gene ID (NCBI) 6119
RRIDAB_2269682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPA3 antibody 10692-1-APDownload protocol
IHC protocol for RPA3 antibody 10692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission

Authors - James Glover
View Article