Validation Data Gallery
|Positive WB detected in||HeLa cells, MCF-7 cells, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10410-1-AP targets RFC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RFC2 fusion protein Ag0663|
|Full Name||replication factor C (activator 1) 2, 40kDa|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC002813|
|Gene ID (NCBI)||5982|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).
RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.
Proc Natl Acad Sci U S A
Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.
Mol Syst Biol
The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lei (Verified Customer) (11-29-2018)
This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.