RFC2 Polyclonal antibody
RFC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10410-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-3 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
10410-1-AP targets RFC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RFC2 fusion protein Ag0663 |
| Full Name | replication factor C (activator 1) 2, 40kDa |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC002813 |
| Gene Symbol | RFC2 |
| Gene ID (NCBI) | 5982 |
| RRID | AB_2285035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFC2 antibody 10410-1-AP | Download protocol |
| IF protocol for RFC2 antibody 10410-1-AP | Download protocol |
| IP protocol for RFC2 antibody 10410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Syst Biol The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation. | ||
Proc Natl Acad Sci U S A Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. | ||
Sci Rep RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas. | ||
bioRxiv Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination | ||
Cell Rep Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lei (Verified Customer) (11-29-2018) | This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.
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