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REDD1 specific Polyclonal antibody
REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
269
Cat no : 10638-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells, SH-SY5Y cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, rabbit, squirrel, gerbil |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | REDD1 specific fusion protein Ag0965 |
| Full Name | DNA-damage-inducible transcript 4 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC007714 |
| Gene Symbol | REDD1/DDIT4 |
| Gene ID (NCBI) | 54541 |
| RRID | AB_2245711 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
| IHC protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
| IF protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
| IP protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. | ||
Nat Immunol The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. | ||
Acta Neuropathol MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Yuki (Verified Customer) (11-10-2022) | This product was used for IHC in human tissue.
 |
FH Stephane (Verified Customer) (11-26-2020) | The antibody performs great on human cell extract. It detects specific bands with the REDD1 band resolving around 30-35 kD.
 |
FH Jamal (Verified Customer) (09-17-2019) | This product was used for Western Blotting in mouse cortex tissue.
|
FH Bradley (Verified Customer) (08-19-2019) | I have used this antibody on numerous occasions with success. The antibody performs great on human cell extract, as evidenced by a single immunoreactive band. The antibody performs moderately well on mouse skeletal muscle extract as it detects many non-specific bands with the REDD1 band resolving around 32-35 kD (specificity determined using REDD1-/- samples). The antibody is capable of detecting large changes in REDD1 protein in mouse skeletal muscle (i.e. induction following dexamethasone treatment), but smaller changes are difficult to assess in this tissue. This antibody will detect smaller changes in human cell lines such as HEK 293 or Hela cells.
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