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  • Featured Product
  • KD/KO Validated

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11329-2-AP

Synonyms

Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis using 11329-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of A431 using 11329-2-AP

    WB result of RAB35 antibody (11329-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB35 transfected A431 cells.

  • Western Blot (WB) analysis of MCF-7 cells using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of MCF-7 using 11329-2-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of A375 cells using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of A375 using 11329-2-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of HeLa using 11329-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of RAW 264.7 using 11329-2-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of human brain tissue using RAB35 Polyclonal antibody (11329-2-AP)

    WB analysis of human brain using 11329-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IP experiment of mouse brain using 11329-2-AP

    IP result of anti-RAB35 (IP:11329-2-AP, 4ug; Detection:11329-2-AP 1:600) with mouse brain tissue lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human breast cancer using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human breast cancer using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human liver using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human liver using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human brain using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB35 Polyclonal antibody (11329-2-AP)

    IHC staining of human brain using 11329-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using RAB35 Polyclonal antibody (11329-2-AP)

    IF Staining of U-87 MG using 11329-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB35 antibody (11329-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11329-2-AP targets RAB35 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB35 fusion protein Ag1872
Full Name RAB35, member RAS oncogene family
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC015931
Gene Symbol RAB35
Gene ID (NCBI) 11021
RRIDAB_2238179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

Protocols

Product Specific Protocols
WB protocol for RAB35 antibody 11329-2-APDownload protocol
IHC protocol for RAB35 antibody 11329-2-APDownload protocol
IF protocol for RAB35 antibody 11329-2-APDownload protocol
IP protocol for RAB35 antibody 11329-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Identification of an oncogenic RAB protein.

Authors - Douglas B Wheeler
  • KD Validated
View Article
humanWB

Cell Metab

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Authors - Chaitali Mukherjee
View Article
humanWB

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
View Article
humanWB

Nat Cell Biol

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

Authors - Alexander Wallroth
View Article
mouseWB,IF

Sci Adv

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Authors - Luis Bonet-Ponce
View Article
human,mouseWB,IHC,IF,CoIP

Nat Commun

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Authors - Corallino Salvatore
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Xin (Verified Customer) (03-15-2022)

A clean antibody to probe Rab35 with the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human postmortem brain lysate
RAB35 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:11329-2-AP)
FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB35 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11329-2-AP)
FH

Mayur (Verified Customer) (01-14-2019)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain tissue (Cortex)
RAB35 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain tissue (Cortex) (Cat no:11329-2-AP)