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PKC Gamma Monoclonal antibody

PKC Gamma Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F4B9

Cat no : 66429-1-Ig

Synonyms

PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Gamma Monoclonal antibody (66429-1-Ig)

    WB analysis using 66429-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IF Staining of SH-SY5Y using 66429-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    FC experiment of SH-SY5Y using 66429-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (66429-1-Ig, Clone:2F4B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66429-1-Ig targets PKC Gamma in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PKC Gamma fusion protein Ag5910
Full Name protein kinase C, gamma
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC047876
Gene Symbol PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2881800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

Protocols

Product Specific Protocols
WB protocol for PKC Gamma antibody 66429-1-IgDownload protocol
IHC protocol for PKC Gamma antibody 66429-1-IgDownload protocol
IF protocol for PKC Gamma antibody 66429-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols