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VPS15 Polyclonal antibody

VPS15 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17894-1-AP

Synonyms

PI3K p150, PIK3R4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase regulatory subunit 4, Phosphoinositide 3-kinase adaptor protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis of HepG2 using 17894-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis using 17894-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS15 Polyclonal antibody (17894-1-AP)

    IF Staining of HeLa using 17894-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17894-1-AP targets VPS15 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS15 fusion protein Ag12221
Full Name phosphoinositide-3-kinase, regulatory subunit 4
Calculated Molecular Weight 1358 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC110318
Gene Symbol PIK3R4
Gene ID (NCBI) 30849
RRIDAB_2163630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

Protocols

Product Specific Protocols
WB protocol for VPS15 antibody 17894-1-APDownload protocol
IF protocol for VPS15 antibody 17894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.

Authors - Thomas Gstrein
View Article
humanWB

Nat Commun

Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.

Authors - Shirley Tremel
View Article
IP,CoIP

Nat Commun

IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.

Authors - Fuqing Hu
View Article
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
View Article
ratWB

J Hazard Mater

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Authors - Yiyuan Kang
View Article

Cancer Lett

Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth.

Authors - Meng Zhang
View Article