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PHACTR1 Polyclonal antibody

PHACTR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23446-1-AP

Synonyms

RPEL1, RPEL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PHACTR1 Polyclonal antibody (23446-1-AP)

    WB analysis using 23446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23446-1-AP (PHACTR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PHACTR1 Polyclonal antibody (23446-1-AP)

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PHACTR1 Polyclonal antibody (23446-1-AP)

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PHACTR1 Polyclonal antibody (23446-1-AP)

    IF Staining of mouse brain using 23446-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PHACTR1 antibody (23446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23446-1-AP targets PHACTR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACTR1 fusion protein Ag20125
Full Name phosphatase and actin regulator 1
Calculated Molecular Weight 580 aa, 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC101526
Gene Symbol PHACTR1
Gene ID (NCBI) 221692
RRIDAB_2879281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PHACTR1 antibody 23446-1-APDownload protocol
IHC protocol for PHACTR1 antibody 23446-1-APDownload protocol
IF protocol for PHACTR1 antibody 23446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pediatr

Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS

Authors - A Kaitlyn Victor
View Article