Pepsinogen I Polyclonal antibody

Pepsinogen I Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17330-1-AP

Synonyms

Pepsin A, pepsinogen 5, group I (pepsinogen A), Pepsinogen I, PGA3, PGA4, PGA5



Tested Applications

Positive WB detected inBGC-823 cells, SGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17330-1-AP targets Pepsinogen I in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pepsinogen I fusion protein Ag11309
Full Name pepsinogen 5, group I (pepsinogen A)
Calculated Molecular Weight 388 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC029055
Gene Symbol PGA5
Gene ID (NCBI) 5222
RRIDAB_11042599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene encodes a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).

Protocols

Product Specific Protocols
WB protocol for Pepsinogen I antibody 17330-1-APDownload protocol
IHC protocol for Pepsinogen I antibody 17330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.

Authors - Jie Liu
ratIF

PLoS One

The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival.

Authors - Pål Vange
humanIHC

Hum Pathol

Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer.

Authors - Yu Zhang
mouseIHC

Dev Dyn

Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling

Authors - Wen Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

FAISAL (Verified Customer) (05-03-2021)

I found this antibody very good and give very good and consistent result in low concentration.

  • Applications: Western Blot, Immunohistochemistry
  • Cell Tissue Type: cell and tissue