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  • Featured Product
  • KD/KO Validated

Pyruvate Carboxylase Polyclonal antibody

Pyruvate Carboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16588-1-AP

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    WB analysis using 16588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    WB analysis of HepG2 using 16588-1-AP

    WB result of Pyruvate Carboxylase antibody (16588-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IP experiment of HepG2 using 16588-1-AP

    IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:1000) with HepG2 cells lysate 2640 ug.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IP experiment of mouse liver using 16588-1-AP

    IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human liver cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human liver cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human liver cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human breast cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human breast cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IHC staining of human liver cancer using 16588-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IF Staining of HepG2 using 16588-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

    IF Staining of HepG2 using 16588-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pyruvate Carboxylase fusion protein Ag8857
Full Name pyruvate carboxylase
Calculated Molecular Weight 1178 aa, 130 kDa
Observed Molecular Weight 125-130 kDa
GenBank Accession NumberBC011617
Gene Symbol Pyruvate Carboxylase
Gene ID (NCBI) 5091
RRIDAB_1851513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Protocols

Product Specific Protocols
WB protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IHC protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IF protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IP protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells

Authors - Kevin M Rattigan
View Article
mouseWB

Nat Commun

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Authors - Jin-Ho Koh
View Article
humanIP

Nat Commun

The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.

Authors - Alastair G Kerr
View Article
humanWB

Nat Commun

SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.

Authors - Peter J Mullen
View Article
mouseWB

Cancer Res

A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.

Authors - Jennifer G Gill
View Article
mouseWB

Elife

Differential requirements for mitochondrial electron transport chain components in the adult murine liver

Authors - Nicholas P Lesner
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Kevin (Verified Customer) (02-10-2022)

This worked as well as other much more expensive antibodies from differenet suppliers

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562