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NPTX2 Polyclonal antibody
NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10889-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells, PC-3 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 6 publications below |
| IF | See 9 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10889-1-AP targets NPTX2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NPTX2 fusion protein Ag1326 |
| Full Name | neuronal pentraxin II |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47-50 kDa |
| GenBank Accession Number | BC009924 |
| Gene Symbol | NPTX2 |
| Gene ID (NCBI) | 4885 |
| RRID | AB_2153875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| IHC protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| IF protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| IP protocol for NPTX2 antibody 10889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Invest Dermatol NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail | ||
Neuropsychopharmacology NPTX2 is a key component in the regulation of anxiety.
| ||
Front Immunol Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice. | ||
J Neurochem Quantitative proteomic analysis of the frontal cortex in Alzheimer's disease. | ||
J Cancer Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer. | ||
Mol Biol Rep The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xin (Verified Customer) (10-10-2022) | Very good with WB to detect endogenous NPTX2 with a clean and clear band with the right MW.
 |
FH Jerome (Verified Customer) (05-07-2019) | This protein should be in the secretory pathway but instead the antibody stains the nucleus
 |
