• Featured Product
  • KD/KO Validated

NDUFV1 Polyclonal antibody

NDUFV1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11238-1-AP

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells, mouse heart tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11238-1-AP targets NDUFV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV1 fusion protein Ag1764
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC015645
Gene Symbol NDUFV1
Gene ID (NCBI) 4723
RRIDAB_2149040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Protocols

Product Specific Protocols
WB protocol for NDUFV1 antibody 11238-1-APDownload protocol
IHC protocol for NDUFV1 antibody 11238-1-APDownload protocol
IP protocol for NDUFV1 antibody 11238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
humanWB

Nature

Accessory subunits are integral for assembly and function of human mitochondrial complex I.

Authors - David A Stroud
humanWB

Mol Cell

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.

Authors - Yilin Kang
human,mouseWB

Nat Commun

A salvage pathway maintains highly functional respiratory complex I.

Authors - Karolina Szczepanowska
mouseWB

Brain

CLPP deficiency ameliorates neurodegeneration caused by impaired mitochondrial protein synthesis.

Authors - Anastasia Rumyantseva
mouseWB

Dev Cell

Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.

Authors - Rani Ojha