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Neprilysin/CD10 Polyclonal antibody

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 18008-1-AP

Synonyms

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    WB analysis using 18008-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    WB analysis of mouse kidney using 18008-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    WB analysis of human kidney using 18008-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    WB analysis of rat kidney using 18008-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    WB analysis of mouse kidney using 18008-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IP experiment of Raji using 18008-1-AP

    IP result of anti-Neprilysin/CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human renal cell carcinoma using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human renal cell carcinoma using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MME,CD10 Polyclonal antibody (18008-1-AP)

    IHC staining of human kidney using 18008-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IF Staining of human kidney using 18008-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Neprilysin/CD10 Polyclonal antibody (18008-1-AP)

    IF Staining of human kidney using 18008-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18008-1-AP targets Neprilysin/CD10 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neprilysin/CD10 fusion protein Ag12506
Full Name membrane metallo-endopeptidase
Calculated Molecular Weight80aa,9 kDa; 750aa,85 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC106070
Gene Symbol CD10
Gene ID (NCBI) 4311
RRIDAB_2146541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IHC protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IF protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IP protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

Authors - Rui Zhou
View Article
humanWB

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu
View Article
humanWB

Int J Biol Macromol

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Authors - Hui Zeng
View Article
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue
View Article
humanIHC

BMC Genomics

Genetic control of renal tumorigenesis by the mouse Rtm1 locus.

Authors - Jensen José Ricardo JR
View Article
mouseWB

J Alzheimers Dis

Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.

Authors - Ling-Li Lv
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

  • Applications: Western Blot