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Biotin Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for IHC, FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IHC, FC

Conjugate

Biotin

CloneNo.

6D4

Cat no : Biotin-65161

Synonyms

MICA, MIC-A, MIC A, 6D4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human colon using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    IHC staining of human colon using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161)

    FC experiment of HeLa using Biotin-65161

    1x10^6 HeLa cells were surface stained with 0.5 ug Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161, Clone:6D4) or isotype control, and SA-PE. Cells were not fixed.

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65161 targets MICA/MICB in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Transfected C1R cells expressing MICA
Full Name MHC class I polypeptide-related sequence A
Calculated Molecular Weight 383 aa, 43 kDa
GenBank Accession NumberBC016929
Gene Symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_2883067
Conjugate Biotin
Form Liquid
Purification MethodThe purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Protocols

Product Specific Protocols
IHC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
FC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
Standard Protocols
Click here to view our Standard Protocols