- Featured Product
- KD/KO Validated
MFN1 Polyclonal antibody
MFN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
271
Cat no : 13798-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HSC-T6 cells, T-47D cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 258 publications below |
| IHC | See 13 publications below |
| IF | See 14 publications below |
| IP | See 1 publications below |
Product Information
13798-1-AP targets MFN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, hamster, goat, ducks, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MFN1 fusion protein Ag4762 |
| Full Name | mitofusin 1 |
| Calculated Molecular Weight | 741 aa, 84 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC040557 |
| Gene Symbol | MFN1 |
| Gene ID (NCBI) | 55669 |
| RRID | AB_2266318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MFN1 antibody 13798-1-AP | Download protocol |
| IHC protocol for MFN1 antibody 13798-1-AP | Download protocol |
| IF protocol for MFN1 antibody 13798-1-AP | Download protocol |
| IP protocol for MFN1 antibody 13798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Cell Mitocytosis, a migrasome-mediated mitochondrial quality-control process.
| ||
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH S (Verified Customer) (09-23-2024) | Good
 |
FH Ruiting (Verified Customer) (09-18-2020) | Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.
|
FH Kishor (Verified Customer) (01-30-2019) | I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue
|
