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LIMPII Polyclonal antibody

LIMPII Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27102-1-AP

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 27102-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 27102-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 27102-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25908 Product name: Recombinant human LIMPII protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-433 aa of BC021892 Sequence: NGTDGDSFHPLITKDEVLYVFPSDFCRSVYITFSDYESVQGLPAFRYKVPAEILANTSDNAGFCIPEGNCLGSGVLNVSICKNGAPIIMSFPHFYQADERFVSAIEGMHPNQEDHETFVDINPLTGIILKAAKRFQINIYVKKLDDFVETGDIRTMVFPVMYLNESVHIDKETASRLKSMINTTL Predict reactive species
Full Name	scavenger receptor class B, member 2
Calculated Molecular Weight	478 aa, 54 kDa
Observed Molecular Weight	75-85 kDa
GenBank Accession Number	BC021892
Gene Symbol	SCARB2
Gene ID (NCBI)	950
RRID	AB_2880756
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14108
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for LIMPII antibody 27102-1-AP	Download protocol
WB protocol for LIMPII antibody 27102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

LIMPII Polyclonal antibody (Cat# 27102-1-AP) has 12 total citations. These appear in journals including Nature, Autophagy, Advanced Science, PLoS Pathogens, Journal of Virology, Viruses, Virus Research, Infectious Genetics and Evolution, Journal of Neurovirology, Virologica Sinica, and bioRxiv. The research fields span cancer biology (ferroptosis, melanoma metastasis), cell biology (Golgi architecture, lysosomal calcium signaling), virology (HIV-1, enterovirus EV71/CA16, PRRSV), and neurovirology (blood-brain barrier permeability).

Species	Application	Title
human,mouse	WB	Nature Lymph node environment drives FSP1 targetability in metastasizing melanoma Authors - Mario Palma View Article
human	WB	Nature Activation of lysosomal iron triggers ferroptosis in cancer Authors - Tatiana Cañeque View Article
human	WB	Autophagy A role for CASM in the repair of damaged Golgi architecture. Authors - Seeun Oh View Article
human	WB	Adv Sci (Weinh) Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium Authors - Meng-Yuan Zhu View Article
human	WB	PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness Authors - Elena Sugrue View Article
pig	WB	Virol Sin In vivo quantitative proteomic analysis of porcine alveolar macrophages in PRRSV-infected pigs Authors - Ying Wei KD Validated View Article