• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LDB1 Polyclonal antibody

LDB1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16650-1-AP

Synonyms

CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LDB1 Polyclonal antibody (16650-1-AP)

    WB analysis using 16650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16650-1-AP (LDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using LDB1 Polyclonal antibody (16650-1-AP)

    WB analysis of HEK-293 using 16650-1-AP

    WB result of LDB1 antibody (16650-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDB1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using LDB1 Polyclonal antibody (16650-1-AP)

    IP experiment of MCF-7 using 16650-1-AP

    IP result of anti-LDB1 (IP:16650-1-AP, 4ug; Detection:16650-1-AP 1:1500) with MCF-7 cells lysate 1000 ug.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16650-1-AP targets LDB1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDB1 fusion protein Ag10082
Full Name LIM domain binding 1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC000482
Gene Symbol LDB1
Gene ID (NCBI) 8861
RRIDAB_2137010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1. This antibody reacts with isoform 1.

Protocols

Product Specific Protocols
WB protocol for LDB1 antibody 16650-1-APDownload protocol
IP protocol for LDB1 antibody 16650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols