Tested Applications
| Positive WB detected in | Human peripheral blood platelets, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18309-1-AP targets CD61 / Integrin beta 3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC127667 |
| Gene Symbol | Integrin beta 3 |
| Gene ID (NCBI) | 3690 |
| RRID | AB_2128759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05106 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CD61 / Integrin beta 3 antibody 18309-1-AP | Download protocol |
| WB protocol for CD61 / Integrin beta 3 antibody 18309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ITGB3 antibody (Cat# 18309-1-AP) has 87 total citations published in over 70 journals, including Nature Communications, Science Bulletin, Journal of Clinical Investigation, Advanced Science, Biomaterials, and Cell Death Discovery. Associated research fields encompass platelet biology and thrombosis, cardiovascular disease, bone metabolism and osteoporosis, oncology (melanoma, breast, prostate cancer), reproductive biology, diabetes complications, inflammation and immunology, nanomedicine, fibrosis, and neurology.
| Species | Application | Title |
|---|---|---|
Bioact Mater Chiral Fe(3)O(4)/GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis. | ||
Sci Bull (Beijing) Engineered platelets-based nano-aircraft system for precise tumor chemo-immunotherapy with graded drug delivery and self-recognized tumor targeting | ||
Autophagy Cardiac fibroblast-derived CCN1 aggravates diabetic cardiomyopathy through ITGAV-ITGB1/integrin αvβ1-mediated autophagy inhibition. | ||
Nat Commun Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis | ||
Nat Commun Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo | ||
Reviews
What customers say
All three reviews are 5-star ratings from Western Blot users. Reviewers consistently report clear, strong bands at the expected ~100–110 kDa molecular weight when detecting ITGB3. Tested samples included mouse lysed blood and liver tissue, with dilutions of 1:500 and 1:1000. Users strongly recommend this antibody for ITGB3 detection by Western Blot, describing the results as reliable and high quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-07-2025) | BEST FOR WESTERN
|
Sathyanarayani (Verified Customer) (04-05-2023) | I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.
|
Sathyanarayani (Verified Customer) (04-05-2023) | The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.
|
