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- KD/KO Validated
INO80 Polyclonal antibody
INO80 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 24819-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| ChIP | See 1 publications below |
Product Information
The immunogen of 24819-1-AP is INO80 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INO80 fusion protein Ag20309 |
| Full Name | INO80 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 1556 aa, 177 kDa |
| Observed Molecular Weight | 177 kDa, 150 kDa |
| GenBank Accession Number | BC146785 |
| Gene Symbol | INO80 |
| Gene ID (NCBI) | 54617 |
| RRID | AB_2879741 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INO80 antibody 24819-1-AP | Download protocol |
| IHC protocol for INO80 antibody 24819-1-AP | Download protocol |
| IP protocol for INO80 antibody 24819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. | ||
Genes Dev INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma. | ||
Int J Mol Sci Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells
| ||
Sci Rep Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.
| ||
FEBS J The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.
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