• Featured Product
  • KD/KO Validated

IDH1 Polyclonal antibody

IDH1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12332-1-AP

Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD



Tested Applications

Positive WB detected inHEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12332-1-AP targets IDH1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH1 fusion protein Ag2986
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC012846
Gene symbol IDH1
Gene ID (NCBI) 3417
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Protocols

Product Specific Protocols
WB protocol for IDH1 antibody 12332-1-APDownload protocol
IF protocol for IDH1 antibody 12332-1-APDownload protocol
IP protocol for IDH1 antibody 12332-1-APDownload protocol
FC protocol for IDH1 antibody 12332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Authors - John P Morris
  • KD Validated
humanWB,IHC

Nature

IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Authors - Lu Chao C
humanWB

Nature

Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Authors - Dang Lenny L
humanWB

Nature

Quantitative flux analysis reveals folate-dependent NADPH production.

Authors - Jing Fan
humanWB

Cancer Cell

Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.

Authors - Maria E Figueroa
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Umut (Verified Customer) (07-25-2023)

The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U87MG
IDH1 Antibody Western Blot validation (1:1000 dilution) in U87MG (Cat no:12332-1-AP)