• Featured Product
  • KD/KO Validated

IDH1 Polyclonal antibody

IDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12332-1-AP

Synonyms

IDP, IDH 1, IDH, IDCD, EC:1.1.1.42



Tested Applications

Positive WB detected inHEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH1 fusion protein Ag2986
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_2123159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Protocols

Product Specific Protocols
WB protocol for IDH1 antibody 12332-1-APDownload protocol
IHC protocol for IDH1 antibody 12332-1-APDownload protocol
IF protocol for IDH1 antibody 12332-1-APDownload protocol
IP protocol for IDH1 antibody 12332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Authors - John P Morris
  • KD Validated
humanWB,IHC

Nature

IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Authors - Lu Chao C
humanWB

Nature

Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Authors - Dang Lenny L
humanWB

Nature

Quantitative flux analysis reveals folate-dependent NADPH production.

Authors - Jing Fan
humanWB

Cancer Cell

Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.

Authors - Maria E Figueroa
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Umut (Verified Customer) (07-25-2023)

The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U87MG
IDH1 Antibody Western Blot validation (1:1000 dilution) in U87MG (Cat no:12332-1-AP)