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  • Featured Product
  • KD/KO Validated

GCLM Polyclonal antibody

GCLM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14241-1-AP

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLM Polyclonal antibody (14241-1-AP)

    WB analysis using 14241-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GCLM Polyclonal antibody (14241-1-AP)

    WB analysis of HeLa using 14241-1-AP

    WB result of GCLM antibody (14241-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using GCLM Polyclonal antibody (14241-1-AP)

    WB analysis using 14241-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using GCLM Polyclonal antibody (14241-1-AP)

    IP experiment of A431 using 14241-1-AP

    IP result of anti-GCLM (IP:14241-1-AP, 4ug; Detection:14241-1-AP 1:2000) with A431 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human liver cancer using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human liver cancer using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human liver cancer using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human liver cancer using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human skeletal muscle using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GCLM Polyclonal antibody (14241-1-AP)

    IHC staining of human skeletal muscle using 14241-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GCLM Polyclonal antibody (14241-1-AP)

    IF Staining of HepG2 using 14241-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GCLM antibody (14241-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using GCLM Polyclonal antibody (14241-1-AP)

    FC experiment of HepG2 using 14241-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GCLM Polyclonal antibody (14241-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue, HepG2 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman liver cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14241-1-AP targets GCLM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCLM fusion protein Ag5497
Full Name glutamate-cysteine ligase, modifier subunit
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC041809
Gene Symbol GCLM
Gene ID (NCBI) 2730
RRIDAB_2107832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Protocols

Product Specific Protocols
WB protocol for GCLM antibody 14241-1-APDownload protocol
IHC protocol for GCLM antibody 14241-1-APDownload protocol
IF protocol for GCLM antibody 14241-1-APDownload protocol
IP protocol for GCLM antibody 14241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
View Article
mouseWB

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
View Article
mouseWB

Hepatology

Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.

Authors - Yu Sun
View Article
mouseWB

Acta Pharm Sin B

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

Authors - Yujun Zhou
View Article
mouseWB

Sci Adv

Immunomodulatory actions of a kynurenine-derived endogenous electrophile.

Authors - Mara Carreño
View Article
mouseWB

Nat Commun

Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.

Authors - Yao Wei
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hua (Verified Customer) (01-17-2023)

Good antibody for WB. There are some non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hela, human liver and mouse liver
GCLM Antibody Western Blot validation (1:2000 dilution) in Hela, human liver and mouse liver (Cat no:14241-1-AP)