• Featured Product
  • KD/KO Validated

GCLC Polyclonal antibody

GCLC Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12601-1-AP

Synonyms

Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC



Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, L02 cells, HEK-293 cells, Raji cells, mouse lung tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12601-1-AP targets GCLC in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCLC fusion protein Ag3252
Full Name glutamate-cysteine ligase, catalytic subunit
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC022487
Gene symbol GCLC
Gene ID (NCBI) 2729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Protocols

Product Specific Protocols
WB protocol for GCLC antibody 12601-1-APDownload protocol
IHC protocol for GCLC antibody 12601-1-APDownload protocol
IP protocol for GCLC antibody 12601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
  • KO Validated
humanWB

Cell

Differential Requirements for eIF4E Dose in Normal Development and Cancer.

Authors - Morgan L Truitt
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
mouseWB

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
mouseWB

Cell Metab

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Authors - Joni Nikkanen
mouseWB

Hepatology

Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.

Authors - Yu Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

AIK SENG (Verified Customer) (01-10-2023)

Clear & crisp bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Colorectal cancer