Tested Applications
| Positive WB detected in | Jurkat cells, L02 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Ramos cells, mouse lung tissue, rat lung tissue, C6 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12601-1-AP targets GCLC in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3252 Product name: Recombinant human GCLC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC022487 Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV Predict reactive species |
| Full Name | glutamate-cysteine ligase, catalytic subunit |
| Calculated Molecular Weight | 637 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC022487 |
| Gene Symbol | GCLC |
| Gene ID (NCBI) | 2729 |
| RRID | AB_2278734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family. It is also known as GLCL and GLCLC. It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis. GCLC may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCLC antibody 12601-1-AP | Download protocol |
| IP protocol for GCLC antibody 12601-1-AP | Download protocol |
| WB protocol for GCLC antibody 12601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCLC Polyclonal antibody (Cat# 12601-1-AP) has 206 citations, appearing in high-impact journals including Nature, Science, Cell, Cell Metabolism, Nature Communications, PNAS, Cell Reports, and Journal of Clinical Investigation. Research fields center on glutathione metabolism, ferroptosis, oxidative stress, and the Keap1/Nrf2 antioxidant pathway, spanning liver injury, cancer, neurodegeneration, and inflammation. Applications are predominantly Western blot, with some IHC and IF.
| Species | Application | Title |
|---|---|---|
Nature SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.
| ||
Cell Metab Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex | ||
Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sai Sindhura (Verified Customer) (01-08-2026) | Antibody is giving sharp bands
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Zach (Verified Customer) (08-29-2025) | Our IHC core recommend me to get this antibody for IHC staining in human samples, pretty good experience.
|
AIK SENG (Verified Customer) (01-10-2023) | Clear & crisp bands
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