• Featured Product
  • KD/KO Validated

FURIN Polyclonal antibody

FURIN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 18413-1-AP


Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18413-1-AP targets FURIN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FURIN fusion protein Ag13261
Full Name furin (paired basic amino acid cleaving enzyme)
Calculated molecular weight 87 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC012181
Gene symbol FURIN
Gene ID (NCBI) 5045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and the shed furin at 90 kDa.


Product Specific Protocols
WB protocol for FURIN antibody 18413-1-APDownload protocol
IHC protocol for FURIN antibody 18413-1-APDownload protocol
IP protocol for FURIN antibody 18413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.

Authors - Diana Farhat


Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.

Authors - Huizhi Wang

Br J Cancer

Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.

Authors - Diana Farhat
  • KD Validated

J Virol

Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry

Authors - Timothy J Smith
  • KO Validated


SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization.

Authors - Jia-Xing Sun

Biochem Pharmacol

Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.

Authors - Jordan M Buck


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: 293T, HeLa