Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, mouse ovary tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14768-1-AP targets FEN1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6552 Product name: Recombinant human FEN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-380 aa of BC000323 Sequence: MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK Predict reactive species |
| Full Name | flap structure-specific endonuclease 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000323 |
| Gene Symbol | FEN1 |
| Gene ID (NCBI) | 2237 |
| RRID | AB_2102780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P39748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FEN1 antibody 14768-1-AP | Download protocol |
| IHC protocol for FEN1 antibody 14768-1-AP | Download protocol |
| IP protocol for FEN1 antibody 14768-1-AP | Download protocol |
| WB protocol for FEN1 antibody 14768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FEN1 antibody (Cat# 14768-1-AP) has accumulated 23 citations across 22 journals, including Cell, Nature Communications, Nucleic Acids Research, Advanced Science, Cell Reports, Journal of Translational Medicine, and Science Reports. Associated research fields span DNA damage repair, tumorigenesis and cancer biology, ferroptosis, pyroptosis, cellular senescence, HIV integration, genome stability, neurodegeneration, pulmonary fibrosis, and chemo-immunotherapy.
| Species | Application | Title |
|---|---|---|
Cell Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors | ||
Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. | ||
Nucleic Acids Res Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathways. | ||
Adv Sci (Weinh) CypD Dependent mPTP Opening Is Crucial for Oxidized Mitochondrial DNA Release in Ferroptosis. | ||
Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia | ||
Cell Death Dis DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1 |
