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NSE Polyclonal antibody

NSE Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10149-1-AP

Synonyms

ENO2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue, U-251 cells, SH-SY5Y cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human lung tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10149-1-AP targets NSE in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSE fusion protein Ag0196
Full Name enolase 2 (gamma, neuronal)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC002745
Gene Symbol ENO2
Gene ID (NCBI) 2026
RRIDAB_2099180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Protocols

Product Specific Protocols
WB protocol for NSE antibody 10149-1-APDownload protocol
IHC protocol for NSE antibody 10149-1-APDownload protocol
IF protocol for NSE antibody 10149-1-APDownload protocol
IP protocol for NSE antibody 10149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseWB

Mater Today Bio

In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.

Authors - Dan Zhang
mouseWB

Mater Today Bio

Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.

Authors - Luyu Wang
humanWB

Aging (Albany NY)

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.

Authors - Fang Li
goatIF

Cell Cycle

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.

Authors - Zhimin Wang
humanWB

Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang