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  • Featured Product
  • KD/KO Validated

EIF2AK1 Polyclonal antibody

EIF2AK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20499-1-AP

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2AK1 Polyclonal antibody (20499-1-AP)

    WB analysis using 20499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EIF2AK1 Polyclonal antibody (20499-1-AP)

    WB analysis of HEK-293 using 20499-1-AP

    WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HepG2 cells using EIF2AK1 Polyclonal antibody (20499-1-AP)

    WB analysis of HepG2 using 20499-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Polyclonal antibody (20499-1-AP)

    IHC staining of human breast cancer using 20499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Polyclonal antibody (20499-1-AP)

    IHC staining of human breast cancer using 20499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF2AK1 Polyclonal antibody (20499-1-AP)

    IF Staining of HepG2 using 20499-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20499-1-AP targets EIF2AK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK1 fusion protein Ag14236
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_10697665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Protocols

Product Specific Protocols
WB protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IHC protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IF protocol for EIF2AK1 antibody 20499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler
View Article
humanWB

Science

Human NLRP1 is a sensor for double-stranded RNA.

Authors - Stefan Bauernfried
View Article
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
  • KD Validated
View Article
humanWB

Mol Cell

Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.

Authors - Jack Chun-Chieh Hsu
View Article
humanWB,IP

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
View Article
WB

Nat Commun

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Authors - Hongtan Wu
View Article