Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20310-1-AP targets DRD5 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14145 Product name: Recombinant human DRD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 351-477 aa of BC009748 Sequence: NSSLNPVIYAFNADFQKVFAQLLGCSHFCSRTPVETVNISNELISYNQDIVFHKEIAAAYIHMMPNAVTPGNREVDNDEEEGPFDRMFQIYQTSPDGDPVAESVWELDCEGEISLDKITPFTPNGFH Predict reactive species |
| Full Name | dopamine receptor D5 |
| Calculated Molecular Weight | 477 aa, 53 kDa |
| Observed Molecular Weight | 50-53 kDa |
| GenBank Accession Number | BC009748 |
| Gene Symbol | DRD5 |
| Gene ID (NCBI) | 1816 |
| RRID | AB_10699880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DRD5 antibody 20310-1-AP | Download protocol |
| WB protocol for DRD5 antibody 20310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
| ||
Int J Mol Sci Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review. | ||
ACS Chem Neurosci Development of a PC12 Cell Based Assay for Screening Catechol-O-methyltransferase Inhibitors | ||
Cell Oncol (Dordr) Activation of dopamine receptor D1 inhibits glioblastoma tumorigenicity by regulating autophagic activity. | ||
J Neurochem Validation of the Targeted Metabolomic Pathway in the Hippocampus and Comparative Analysis with the Prefrontal Cortex of Social Defeat Model Mice. |
