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DOK7 Polyclonal antibody

DOK7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22697-1-AP

Synonyms

C4orf25, CMS1B, docking protein 7, Dok 7, DOK7, Protein Dok 7

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DOK7 Polyclonal antibody (22697-1-AP)

    WB analysis of HEK-293 using 22697-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DOK7 Polyclonal antibody (22697-1-AP)

    WB analysis of Jurkat using 22697-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DOK7 Polyclonal antibody (22697-1-AP)

    WB analysis of MCF-7 using 22697-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DOK7 Polyclonal antibody (22697-1-AP)

    IHC staining of human skeletal muscle using 22697-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DOK7 Polyclonal antibody (22697-1-AP)

    IHC staining of human skeletal muscle using 22697-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22697-1-AP is DOK7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK7 fusion protein Ag18543
Full Name docking protein 7
Calculated Molecular Weight 504 aa, 53 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC131544
Gene Symbol DOK7
Gene ID (NCBI) 285489
RRIDAB_2879152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOK7, also named as C4orf25, is a MuSK-interacting cytoplasmic protein. DOK7 is essential for neuromuscular synaptogenesis through its interaction with MuSK. It plays an essential role in neuromuscular synaptogenesis. DOK7 acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. DOK7 has some isoforms with MW 53 kDa, 37 kDa, 28 kDa and 64 kDa.

Protocols

Product Specific Protocols
WB protocol for DOK7 antibody 22697-1-APDownload protocol
IHC protocol for DOK7 antibody 22697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols