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  • Featured Product
  • KD/KO Validated

DDB1 Monoclonal antibody

DDB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F6C6

Cat no : 66010-1-Ig

Synonyms

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis using 66010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of A549 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of A549 using 66010-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • DDB1 Antibody WB MCF-7 cells 66010-1-Ig

    WB analysis of MCF-7 cells using 66010-1-Ig

    N/A incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of MCF-7 using 66010-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    IF Staining of MCF-7 using 66010-1-Ig

    Immunofluorescent analysis of fixed MCF-7 cells using 66010-1-Ig (DDB1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66010-1-Ig targets DDB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DDB1 fusion protein Ag17903
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated Molecular Weight 1140 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC011686
Gene Symbol DDB1
Gene ID (NCBI) 1642
RRIDAB_10950859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Protocols

Product Specific Protocols
WB protocol for DDB1 antibody 66010-1-IgDownload protocol
IHC protocol for DDB1 antibody 66010-1-IgDownload protocol
IF protocol for DDB1 antibody 66010-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.

Authors - Yajuan Zhang
  • KD Validated
View Article
humanWB

J Virol

Degradation of SAMHD1 by Vpx is Independent of Uncoating.

Authors - Paula Jáuregui
  • KD Validated
View Article
humanWB

J Virol

Human herpesvirus 6A U4 inhibits proteasomal degradation of amyloid precursor protein.

Authors - Tian Tang
  • KD Validated
View Article