Validation Data Gallery
|Positive WB detected in||mouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14667-1-AP targets Decorin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Decorin fusion protein Ag6328|
|Calculated molecular weight||359 aa, 40 kDa|
|Observed molecular weight||70 kDa, 45-48 kDa|
|GenBank accession number||BC005322|
|Gene ID (NCBI)||1634|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica.
Negative impact of hyperglycaemia on mouse alveolar development.
Stem Cell Res Ther
Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model.
Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.
J Cell Mol Med
Single-cell RNA sequencing analysis to characterize cells and gene expression landscapes in atrial septal defect.
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kenzo (Verified Customer) (08-29-2023)
Antibody is working pretty well for IF on mouse tissue sections.
Huai-Chin (Verified Customer) (10-24-2022)
Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.
balawant (Verified Customer) (07-25-2022)
I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.
Iram (Verified Customer) (06-13-2022)
Excellent antibody for western blotting
Balawant (Verified Customer) (05-08-2022)
I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.
Susmita (Verified Customer) (09-17-2020)
Antibody is working very good for WB