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  • Featured Product
  • KD/KO Validated

Decorin Polyclonal antibody

Decorin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zabrafish and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 14667-1-AP

Synonyms

DCN, CSCD, PGII, PG40, PG S2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Decorin Polyclonal antibody (14667-1-AP)

    WB analysis using 14667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Decorin Polyclonal antibody (14667-1-AP)

    WB analysis of BxPC-3 using 14667-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Decorin Polyclonal antibody (14667-1-AP)

    WB analysis of mouse heart using 14667-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Decorin Polyclonal antibody (14667-1-AP)

    WB analysis of MCF-7 using 14667-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Decorin Polyclonal antibody (14667-1-AP)

    WB analysis of MCF-7 using 14667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human breast cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human skin cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human skin cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human skin cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human normal colon using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human colon cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human colon cancer using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human breast hyperplasia using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human breast hyperplasia using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Decorin Polyclonal antibody (14667-1-AP)

    IHC staining of human breast hyperplasia using 14667-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of zebrafish retina using Decorin Polyclonal antibody (14667-1-AP)

    IF Staining of zebrafish retina using 14667-1-AP

    Immunofluorescence analysis of Decorin polyclonal antibody (14667-1-AP, magenta) staining on tissue sections of the zebrafish retina at 4 days post fertilisation (dpf). Dilution 1:200. Tissue fixed overnight in 4% PFA. DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Decorin Polyclonal antibody (14667-1-AP)

    IF Staining of mouse liver using 14667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Decorin Polyclonal antibody (14667-1-AP)

    IF Staining of mouse liver using 14667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Decorin Polyclonal antibody (14667-1-AP)

    IF Staining of BxPC-3 using 14667-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inzebrafish retina, mouse liver tissue
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14667-1-AP targets Decorin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zabrafish samples.

Tested Reactivity human, mouse, rat, zabrafish
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Decorin fusion protein Ag6328
Full Name decorin
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight70 kDa, 45-48 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_2090265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 14667-1-APDownload protocol
IHC protocol for Decorin antibody 14667-1-APDownload protocol
IF protocol for Decorin antibody 14667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer

Authors - Simeng Hu
View Article
humanIF

Breast Cancer Res

Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors

Authors - Wei Yang
View Article
mouseWB,IF

PLoS Genet

Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica.

Authors - Wing Lee Chan
View Article
mouseIHC, WB

Cell Cycle

Negative impact of hyperglycaemia on mouse alveolar development.

Authors - Mei-Yao He
View Article
ratWB

Stem Cell Res Ther

Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model.

Authors - Zhengzhou Shi
View Article
hamsterWB

Sci Rep

Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.

Authors - Lingling Han
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Kenzo (Verified Customer) (08-29-2023)

Antibody is working pretty well for IF on mouse tissue sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
FH

Huai-Chin (Verified Customer) (10-24-2022)

Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: used on both mouse and human cell lines
FH

balawant (Verified Customer) (07-25-2022)

I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and Caco2 cells
FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for western blotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon cancer cells
FH

Balawant (Verified Customer) (05-08-2022)

I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mice tissue and colon epithelial cells.
FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working very good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB