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DACH1 Polyclonal antibody

DACH1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 10914-1-AP


DACH, DACH1, Dachshund homolog 1, Dachshund-1

Tested Applications

Positive WB detected inH9C2 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10914-1-AP targets DACH1 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACH1 fusion protein Ag1354
Full Name dachshund homolog 1 (Drosophila)
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.


Product Specific Protocols
WB protocol for DACH1 antibody 10914-1-APDownload protocol
IHC protocol for DACH1 antibody 10914-1-APDownload protocol
IF protocol for DACH1 antibody 10914-1-APDownload protocol
IP protocol for DACH1 antibody 10914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (11-02-2021)

This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Heart