COL4A2-specific Polyclonal antibody
COL4A2-specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 55131-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 22 publications below |
| IF | See 14 publications below |
Product Information
55131-1-AP targets COL4A2-specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | collagen, type IV, alpha 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_001846 |
| Gene Symbol | COL4A2 |
| Gene ID (NCBI) | 1284 |
| RRID | AB_2881273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| IHC protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| IF protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. | ||
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Biofabrication Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin. | ||
Biomed Pharmacother Rutaecarpine alleviates inflammation and fibrosis by targeting CK2α in diabetic nephropathy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Samantha (Verified Customer) (05-10-2024) | Clean bands detected in DRG and Spinal cord tissue of mice.
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FH Pankhuri (Verified Customer) (08-22-2022) | Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.
 |
FH Lindsey (Verified Customer) (05-24-2021) | BULLETPROOF ANTIBODY!
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FH Malak (Verified Customer) (02-24-2021) | This antibody works very well
|
FH Joshua (Verified Customer) (04-28-2019) | Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.
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