Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55131-1-AP targets COL4A2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | collagen, type IV, alpha 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_001846 |
| Gene Symbol | COL4A2 |
| Gene ID (NCBI) | 1284 |
| RRID | AB_2881273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| IHC protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| WB protocol for COL4A2 antibody 55131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Rabbit anti-COL4A2 polyclonal antibody (Cat# 55131-1-AP) has accumulated 84 citations. Key journals include Gastroenterology, J Clin Invest, Cell Death Dis, EMBO J, Biomaterials, and Matrix Biol. Research fields predominantly cover diabetic nephropathy/renal fibrosis, liver fibrosis, extracellular matrix biology, cancer (breast, hepatocellular, bladder), tissue engineering/biofabrication, cardiovascular disease, and ocular pathology. Cited applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Gastroenterology Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis | ||
J Exp Clin Cancer Res Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancer. | ||
J Clin Invest Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma | ||
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Cell Death Dis LincRNA 1700020I14Rik alleviates cell proliferation and fibrosis in diabetic nephropathy via miR-34a-5p/Sirt1/HIF-1α signaling. | ||
Cell Death Dis Methyl-CpG-binding domain protein 2 contributes to renal fibrosis through promoting polarized M1 macrophages. |
Reviews
What customers say
All five reviewers rated this antibody 4–5 stars, consistently praising its clean, specific staining across Western Blot, Immunofluorescence, and Immunohistochemistry. Users reported sharp bands in mouse DRG and spinal cord tissue, beautiful collagen fiber staining in NSC organoids, and reliable IHC on human kidney FFPE sections. One reviewer simply called it a "bulletproof antibody." Overall, the feedback highlights strong sensitivity, minimal background, and dependable performance across diverse applications and sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Samantha (Verified Customer) (05-10-2024) | Clean bands detected in DRG and Spinal cord tissue of mice.
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Pankhuri (Verified Customer) (08-22-2022) | Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.
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Lindsey (Verified Customer) (05-24-2021) | BULLETPROOF ANTIBODY!
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Malak (Verified Customer) (02-24-2021) | This antibody works very well
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Joshua (Verified Customer) (04-28-2019) | Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.
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